报告题目：Novel Membranes for Responsive Hydrophobic Interaction Chromatography from Experiments and Simulations
报 告 人：Qian Xianghong教授
邀 请 人：王艳教授
Xianghong Qian，阿肯色大学生物医学工程系教授，拥有80多篇同行评议的期刊出版物，获得中国南京大学化学学士学位和美国乔治华盛顿大学理论化学博士学位，随后在德国马普微观结构物理研究所从事凝聚态理论的博士后研究，曾获得2009年NSF职业奖。目前，Xianghong Qian教授的研究兴趣集中在使用互补的实验和理论工具研究膜的分离，生物质催化转化为生物燃料，智能聚合物和蛋白质纯化的基本过程。
Current resin based hydrophobic interaction chromatography (HIC) is a purification operation used in the production of biopharmaceuticals based on differences in hydrophobicity among products and impurities. The current technology can suffer from protein denaturation, low product yields, the possibility of high pressure and lengthy processing time. Membrane based responsive HIC has been investigated previously for protein purification in a bind-and-elute mode to overcome these limitations. The surface of these responsive HIC is grafted with polymeric ligands with tunable hydrophobicity. Poly (N-vinylcaprolactam) (PVCL) is a prototype thermo- and salt- responsive polymer that can switch its conformation between the hydrophilic and hydrophobic states in response to environmental stimuli. However, the binding capacity and recovery of these responsive HIC remain the major bottleneck for the industrial applications. In order to further improve the protein binding capacity and recovery, complementary experimental and computational studies were performed to elucidate the lower critical solution temperature transition (LCST) of PVCL and the effects of substrates with different surface to volume ratios for immobilizing the HIC ligands. Significant improvement in binding capacity and recovery has been achieved.